Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma; Chunyan Liu; Zhenjiang Hou

doi:doi:10.11648/j.sjph.20210901.12

| Peer-Reviewed

Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, Chunyan Liu, Zhenjiang Hou

Published in Science Journal of Public Health (Volume 9, Issue 1)

Received: 16 December 2020 Published: 18 January 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (P<0.05), while Tregs cell proportion, serum TGF-β and Foxp3 mRNA level were all lower than those in the control group (P<0.05). Conclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.

Published in	Science Journal of Public Health (Volume 9, Issue 1)
DOI	10.11648/j.sjph.20210901.12
Page(s)	12-22
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Th17/Treg Cells, Cytokines, Transcription Factors, Iodine Nutritional Status, Hashimoto's Thyroiditis, Thyroid Autoantibodies

References

[1]	Xiang Guangda. Clinical Thyroidology [M]. People's Medical Publishing House, 2013: 200.
[2]	Hu S, Rayman MP. Multiple Nutritional Factors and the Risk of Hashimoto's Thyroiditis [J]. Thyroid. 2017, 27 (5): 597-610.
[3]	Li HX, Xiang N, Hu WK, et al. Relation between therapy options for Graves' disease and the course of Graves' ophthalmopathy: a systematic review and met analysis [J]. J Endocrinol Invest, 2016, 39 (11): 1225-1233.
[4]	Hou ZJ, Mu ZX, Wang CC. Research Progress of Th17/Treg Cells and Their Transcription Factors in Autoimmune Diseases [J]. American Journal of Clinical and Experimental Medicine 2019, 7 (4): 83-92.
[5]	Chinese Medical Association Endocrine Credits “China thyroid disease treatment guidelines” Writing Group. China thyroid disease treatment guidelines-Thyroiditis [J]. Chin J inter Med, 2 008, 47 (9): 784-788.
[6]	Chinese Medical Association Endocrine Credits “China thyroid disease treatment guidelines” Writing Group. China thyroid disease treatment guidelines-iodine deficiency disorders [J]. Journal of Internal Medicine, 2008, 47 (8): 689-690.
[7]	Huang J, Yu PF, Yang ZP, Clinical application of thyroid stimulating hormone and thyroid autoantibodies in the diagnosis of thyroid diseases [J]. Chinese Journal of Control of Endemic Diseases, 2015, 30 (1): 65-66.
[8]	Wang B, Meng JH. Research of thyroid autoantibodies in the diagnosis of Graves' Disease and Hashimoto's Thyroiditis [J]. Chin J Lab Diagn, 2018, 22 (2): 207-209.
[9]	Dong X. The diagnostic value of serum thyroid autoantibodies for autoimmune thyroid disease [J]. ClinMed, 2018, 38 (5): 40-41.
[10]	Yang J, Li Q. Clinical application of thyroid stimulating hormone and thyroid autoantibodies in the diagnosis of thyroid diseases [J]. ProceClin Med, 2018, 27 (6): 424-426.
[11]	Zou GH, Sun FY, Dai JQ, et al. Role of follicular regulatory T cells in the pathogenesis of Hashimoto thyroiditis [J]. Lab Med, 2019, 34 (8): 682-686.
[12]	Wang YG, Yan SL, Zhao SH, et al. Correlation of Hashimoto’s thyroiditis with urine iodine level among the coastal districts in Shandong Province [J]. Chin J Endoc & Meta, 2004, 20 (4): 337-338.
[13]	Ma QQ, Liang QH, Sun L, et al. Expression and significance of CD4⁺ CD45RO⁺ memory T cell in peripheral blood of patients with Hashimoto's thyroiditis [J]. Chinese Journal of Immunology, 2016, 32 (10): 1527-1531.
[14]	Chen YM, Hu FQ, Huang H, et al. The effect of Treg and Th17 cells in Hashimoto’s thyroiditis patiens with normal thyroid function [J] Med J West China, 2018, 30 (10): 1438-1442.
[15]	Chen YX. Correlation between TNF-α and thyroid autoantibodies in hashimoto thyroiditis patients with different iodine intake [D]. Hebei Medical University, 2011.
[16]	Kleinewietfeld M, HaflerDA. The plasticity of human Treg and Th17 cells and its role in autoimmunity [J]. Semin Immunol. 2013, 25 (4): 305-312.
[17]	Singh B, Schwartz JA, Sandroc kC, et al. Modulation of autoimmune diseases by interleukin (IL)-17 producing regulatory T helper (Th17) cells [J]. Indian J Med Res, 2013, 138 (5): 591-594.
[18]	Figueroa-Vega N, Alfonso-Pérez M, Benedicto I, et al. Increased circulating pro-inflammatory cytokines and Th17 lymphocytes in Hashimoto's thyroiditis [J]. J Clin Endocrinol Metab, 2010, 95, (2): 953-962.
[19]	Shi Y, Wang H, Su Z, et al. Differentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis [J]. Scand J Immunol. 2010, 72 (3): 250-255.
[20]	Wang S, Baidoo SE, Liu Y, et al. T cell-derived leptin contributes to increased frequency of T helper type 17 cells in female patients with Hashimoto's thyroiditis [J]. Clin Exp Immunol. 2013, 171 (1): 63-68.
[21]	Xie XY, Xi' er NY, Guo T, et al. The Alteration of the Th1, Th2, Th17, Treg Cells in the Peripheral Blood in Patients with Grave's Disease (GD) and Hashimoto's Thyroiditis with Hypothyroidism. J Radioimmunology, 2011, 24 (3): 290-293.
[22]	Chen WZ. Immune imbalance of Th17/Treg in hashimoto's thyroiditis [D]. Nanchang Unive, 2012.
[23]	Chen ZJ, LIU C, LI Q, et al. The changes and significance of Th17 cells and related cytokines in autoimmune thyroid diseases [J]. Immunological Journal, 2011, 27 (9): 785-788.
[24]	Zhao JY. Detection and significance of Th17 cells in hashimoto's thyroiditis and Graves' disease [D]. Master's Thesis of Jilin University, 2012.
[25]	Sun QK, Chen ZL, LI CH, et al. The signaling abnormalities of IL-6/TGF-β in Th17/Treg cell imbalance in Hashimoto's thyroiditis [J]. Chinese Journal of Endocrinology and Metabolism, 2015, 31 (4): 320-326.
[26]	Xue HB, Ma L, LI YB, et al. Correlation between Treg/Th17 cell and autoimmunity of Hashimoto's thyroiditis [J]. China Journal of Modern Medicine, 2012, 22 (23): 67-71.
[27]	Jin X, Shang XM, Chen HB, et al. Study on dynamic changes and significance of the peripheral blood regulatory T cells (Treg) and Th17 cells in patients with Hashimoto’s thyroiditis [J]. J ClinExper Med, 2018, 17 (19): 2092-2094.
[28]	Ceyla Konca Degertekin, Banu Aktas Yilmaz, Fusun Balos Toruner, et al. Circulating Th17 cytokine levels are altered in Hashimoto’s thyroiditis [J], Cytokine 80 (2016) 13-17.
[29]	Marazuela M, García-López MA, Figueroa-Vega N, et al. Regulatory T cells in human autoimmune thyroid disease [J]. J Clin Endocrinol Metab. 2006, 91 (9): 3639-3646.
[30]	Gao ST, Zhu TN, Zhang YN, et al. The frequency and function of CD4⁺CD25⁺ regulatory T cells in autoimmune thyroid diseases Chin J Immu, 2011, 27 (3): 269-273.
[31]	Mao C, Wang S, Xiao Y, et al. Impairment of regulatory capacity of CD4⁺CD25⁺ regulatory T cells mediated by dendritic cell polarization and hyperthyroidism in Graves' disease [J]. J Immunol. 2011, 186 (8): 4734-4743.
[32]	Zheng LT, Yu SP, Wang N, et al. Regulatory T cells and TGF-βin autoimmune Therole of thyroid disease [J]. Shandong Med 2012, 52 (01): 81-82.
[33]	Yang ZF, Wu F, TANG JZ, et al. Expression of regulatory T cells in patients with hashimoto's thyroiditis and its significance [J]. China General Practice, 2013, 16 (7C): 2477-2479.
[34]	Bi JH, Hao LX, Huang F, et al. The detection and clinical significance of CD4⁺CD25⁺Foxp3⁺ regulatory T cells and IL-10 in peripheral blood of hashimoto thyroiditis patients [J]. Journal of clinical and experimental medicine, 2017, 16 (1): 38-40.
[35]	Glick AB, Wodzinski A, Fu P, et al. Impairment of regulatory T cell function in autoimmune thyroid disease [J]. Thyroid, 2013, 23 (7): 871-878.
[36]	Bao QY, Xu YS, Yang HY. Regulatory T Cells and Endocrine Diseases. Med Reca, 2014, 20 (18): 3278-3280.
[37]	Zhu JF, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations [J]. Annu Rev Immunol. 2010; 28: 445-489.
[38]	Kleinewietfeld M, Hafler DA. The plasticity of human Treg and Th17 cells and its role in autoimmunity [J]. Semin Immunol. 2013, 25 (4): 305-312.
[39]	Pyzik A, Grywalska E, Matyjaszek-Matuszek B, et al. Immune disorders in Hashimoto's thyroiditis: what do we know so far? [J]. J Immunol Res. 2015, 2015: 979167.
[40]	Rodien P, Madec AM, Ruf J, et al. Antibody-dependent cell-mediated cytotoxicity in autoimmune thyroid disease: relationship to antithyroperoxidase antibodies [J]. J ClinEndocrinol Metab. 1996, 81 (7): 2595-2600.
[41]	Xue HB, Yu XR, Ma L, et al. The possible role of CD4⁺CD25^highFoxp3⁺/CD4⁺IL-17A⁺ cell imbalance in the autoimmunity of patients with Hashimoto thyroiditis [J]. Endocrine. 2015, 50 (3): 665-673.
[42]	González-Amaro R, Marazuela M. T regulatory (Treg) and T helper17 (Th17) lymphocytes in thyroid autoimmunity [J]. Endocrine. 2016, 52 (1): 30-38.
[43]	Li C, Ebert PJ, Li QJ. T cell receptor (TCR) and transforming growth factor β (TGF-β) signaling converge on DNA (cytosine-5)-methyltransferase to control forkhead box protein 3 (foxp3) locus methylation and inducible regulatory T cell differentiation [J]. J Biol Chem. 2013, 288 (26): 19127-19139.
[44]	Cai H, Kong W, Zhou T, et al. Radiofrequency ablation versus reresection in treating recurrent hepatocellular carcinoma: a meta-analysis [J]. Medicine, 2014, 93 (22): e122.
[45]	Hirota K, Martin B, Veldhoen M. Development, regulation and functional capacities of Th17 cells [J]. Sem in Immunopathol, 2010, 32 (1): 3-16.
[46]	Yoshimura A, Muto G. TGF-β function in immune suppression [J]. Curr Top MicrobiolImmunol, 2011, 350: 127-147.
[47]	Liu YZ, Tang XY, Tian J, et al. Th17/Treg Cells Imbalance and GITRL Profile in Patients with Hashimoto’s Thyroiditis [J]. International Journal ofMolecular Sciences, 2014, 15, 21674-21686.
[48]	Caturegli P, De Remigis A, Chuang K, et al. Hashimoto'sthyroiditis: celebrating the centennialthrough the lens of the Johns Hopkins hospital surgical pathology records [J]. Thyroid, 2013, 23 (2): 142-150.,
[49]	Latrofa F, Fiore E, Rago T, et al. Iodine contributes to thyroid autoimmunity in humans by unmasking a cryptic epitope on thyroglobulin [J]. J Clin Endocrinol Metab, 2013, 98 (11): E1768-1774.
[50]	Liu M, Zhao L, Gao Y, et al. Epitope recognition patterns of thyroglobulin antibody in sera from patients with Hashimoto' s thyroiditis on different thyroid functional status [J]. ClinExp Immunol, 2012, 170 (3): 283-290.
[51]	Antonelli A, Ferrari SM, Corrado A, et al. Autoimmune thyroid disorders [J]. Autoimmun Rev, 2015, 14 (2): 174-180.
[52]	Haibo Xue, Yuhua Yang, Ying Zhang, et al. Macrophage Migration Inhibitory Factor Interacting withTh17 Cells May Be Involved in the Pathogenesis of Autoimmune Damage in Hashimoto’s Thyroiditis [J]. Mediators of Inflammation 2015 (2): 1-9.
[53]	Rieu M, Richard A, Rosilio M, et al. Effects of thyroid status on thyroid autoimmunity expression in euthyroid and hypothyroid patients with Hashimoto's thyroiditis [J]. ClinEndocrinol. 1994, 40 (4): 529-35.
[54]	Uqba Khan, Ghazanfar H. T. Lymphocytes and Autoimmunity [J]. Int Rev Cell Mol Biol. 2018, 341: 125-168.
[55]	Gao Y, LIU MM, XIU RJ. Advances in the immunological pathogenesis of hashimoto's thyroiditis [J]. Journal of Internal Medicine Concepts & Practice, 2013, 8 (6): 392-396.

Cite This Article

Plain Text BibTeX RIS

APA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. (2021). Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Science Journal of Public Health, 9(1), 12-22. https://doi.org/10.11648/j.sjph.20210901.12

Copy | Download

ACS Style

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci. J. Public Health 2021, 9(1), 12-22. doi: 10.11648/j.sjph.20210901.12

Copy | Download

AMA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci J Public Health. 2021;9(1):12-22. doi: 10.11648/j.sjph.20210901.12

Copy | Download

@article{10.11648/j.sjph.20210901.12,
  author = {Cuicui Wang and Zhaoxin Mu and Yunxia Chen and Jianfeng Liu and Jinqun Ma and Chunyan Liu and Zhenjiang Hou},
  title = {Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {12-22},
  doi = {10.11648/j.sjph.20210901.12},
  url = {https://doi.org/10.11648/j.sjph.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.12},
  abstract = {Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Yunxia Chen
AU  - Jianfeng Liu
AU  - Jinqun Ma
AU  - Chunyan Liu
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.12
DO  - 10.11648/j.sjph.20210901.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 12
EP  - 22
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.12
AB  - Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.
VL  - 9
IS  - 1
ER  -

Copy | Download

Author Information

Cuicui Wang

Department of Medical Technology, Cangzhou Medical College, Cangzhou, China
Zhaoxin Mu

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China
Yunxia Chen

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Jianfeng Liu

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Jinqun Ma

Endocrinology Department, Cangzhou People's Hospital, Cangzhou, China
Chunyan Liu

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China
Zhenjiang Hou

Cangzhou Thyroid Disease Engineering Research Center, Cangzhou Medical College, Cangzhou, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. (2021). Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Science Journal of Public Health, 9(1), 12-22. https://doi.org/10.11648/j.sjph.20210901.12

Copy | Download

ACS Style

Cuicui Wang; Zhaoxin Mu; Yunxia Chen; Jianfeng Liu; Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci. J. Public Health 2021, 9(1), 12-22. doi: 10.11648/j.sjph.20210901.12

Copy | Download

AMA Style

Cuicui Wang, Zhaoxin Mu, Yunxia Chen, Jianfeng Liu, Jinqun Ma, et al. Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status. Sci J Public Health. 2021;9(1):12-22. doi: 10.11648/j.sjph.20210901.12

Copy | Download

@article{10.11648/j.sjph.20210901.12,
  author = {Cuicui Wang and Zhaoxin Mu and Yunxia Chen and Jianfeng Liu and Jinqun Ma and Chunyan Liu and Zhenjiang Hou},
  title = {Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status},
  journal = {Science Journal of Public Health},
  volume = {9},
  number = {1},
  pages = {12-22},
  doi = {10.11648/j.sjph.20210901.12},
  url = {https://doi.org/10.11648/j.sjph.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20210901.12},
  abstract = {Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Study on Th17/Treg Cells and Cytokines in Hashimoto's Thyroiditis with Different Iodine Nutrition Status
AU  - Cuicui Wang
AU  - Zhaoxin Mu
AU  - Yunxia Chen
AU  - Jianfeng Liu
AU  - Jinqun Ma
AU  - Chunyan Liu
AU  - Zhenjiang Hou
Y1  - 2021/01/18
PY  - 2021
N1  - https://doi.org/10.11648/j.sjph.20210901.12
DO  - 10.11648/j.sjph.20210901.12
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 12
EP  - 22
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20210901.12
AB  - Background: The incidence of AITDs increased with the increase of iodine nutrient levels in the population. HT is one of the most common AITDs. The role of thyroid function stratification, antibody titer, Th17/Treg cells and related factors in the pathogenesis of HT under different iodine nutritional conditions is still unclear. Objective: To investigate the correlation between the thyroid function stratification, autoantibody titer and Th17 cells, Treg cells, cytokines and transcription factors in hashimoto thyroiditis patients with different iodine nutritional status. Methods: Thyroid hormone, autoantibody and urinary iodine levels were measured in 100 hashimoto's thyroiditis (HT) patients and 60 healthy subjects by electrochemical immunoluminescence and iodine-catalyzed arseniummethod. Meanwhile, the proportion and ratio of Th17 cells and Tregs cells in peripheral blood mononuclear cells (PBMC) were determined by immunofluorescence labeling and flow cytometry. The qRT-PCR was used to detect the expression of ROR-γt mRNA and Foxp3 mRNA. Serum IL-17 and TGF-β levels were detected by ELISA. Results: Th17 cell proportion, serum IL-17 and ROR-γt mRNA expression levels in PBMC of HT patients with different iodine nutritional status were all higher than those in the control group (PPConclusions: The thyroid function, autoantibodies, Th17/Tregs cell proportion, cytokines and transcription factors of HT patients with different iodine nutrition status were changed, hich were involved in the development and progression of HT.
VL  - 9
IS  - 1
ER  -

Copy | Download